Cyclodextrin-assisted capillary electrophoretic resolution of 1,1′-bi-2-naphthol atropisomers

✍ Scribed by Orfeo Zerbinati; Francesco Trotta

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 90 KB
• Volume: 22
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/1522-2683(200109)22:16<3578::aid-elps3578>3.0.co;2-r

✦ Synopsis

Cyclodextrin-assisted capillary electrophoretic resolution of 1,1'-bi-2-naphthol atropisomers

Native band g-cyclodextrin (CD), neutral b-CD derivatives and ethylcarbonate derivatives of band g-CD were used as stereoselective additives for CD-capillary zone electrophoresis (CZE) resolution of atropisomers of 1,1'-bi-(2-naphthol) (BN). CZE experiments at variable CD concentration allowed calculating binding constants from electrophoretic mobility data, corrected for electroosmotic flow (EOF) and running buffer viscosity variations. The CDs were chosen on the basis of geometric examination of molecular models of BN and CDs that suggested the possibility of inclusion complexes formation. Optimum concentrations, with aqueous 25 mM phosphate running buffer at pH 10.5, 36 cm650 mm capillary and 10 kV applied potential, were 3.6, 3.9, 2.1, 2.2, 1.9 mM for b-CD, g-CD, ethylcarbonate-b-CD, methyl-b-CD and hydroxypropyl-b-CD, respectively.