Cyclic peptide as reference system for b ion structural analysis in the gas phase

✍ Scribed by Xian Chen; Marcus Tirado; Jeffrey D. Steill; Jos Oomens; Nick C. Polfer

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 224 KB
• Volume: 46
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1982

✦ Synopsis

Infrared multiple photon dissociation spectroscopy and hydrogen/deuterium exchange methods are used to confirm the macrocylic structure of a b~6~ peptide fragment by direct comparison with a synthetically made cyclic peptide. The acetylation of the peptide N‐terminus results in the inhibition of the macrocyclic formation, supporting the “head‐to‐tail” cyclization mechanism. Differences in hydrogen/deuterium exchange rates for macrocyclic and oxazalone structure peptide fragments are interpreted to be a result of the complex interplay of multiple basic sites in the peptide fragment, supporting the relay mechanism for deuterium exchange with CH~3~OD. Copyright © 2011 John Wiley & Sons, Ltd.