Cyclic nucleotides and haemoglobin concentration in rabbit bone marrow cell suspensions stimulated by purified erythropoietin
✍ Scribed by Dr. G. Fanò; G. Menchetti; T. Secca; V. Marsili; G. Della Torre
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 547 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0263-6484
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✦ Synopsis
This study was conducted to determine the possible correlations between cyclic nucleotides cyclic adenosine monophosphate (CAMP) and cyclic guanine monophosphate (cGMP), and haemoglobin (Hb) concentration i n nucleated cell suspensions of rabbit bone marrow incubated with erythropoietin (Ep). The levels of cAMP and cGMP were measured following the addition of different E p concentrations to the suspensions. The H b concentration was also measured in suspensions treated with Ep, dibutyryl CAMP (db-CAMP) or dibutyryl cGMP (db-cGMP), respectively. The following results were obtained: (1) upon the addition of 1 IU ml-' Ep, an increase of CAMP levels was related to a n increase in H b concentration; while a decrease of H b concentration was related to an increase of cGMP levels obtained when 0.1 IU ml-' E p was present i n the incubation mixture. (2) A mimetic effect on H b concentration was obtained upon the addition of db-CAMP or db-cGMP to the suspensions. (3) A quantitative correlation was found between the cAMPIcGMP ratio and H b levels in cellular suspensions. This rapport was reviewed with respect to the controls as a decrease i n Hb Concentration when the ratio is less than one and an increase i n H b concentration when the ratio is greater than one.