Cyclic nucleotide activity in gastrointestinal tissues and fluids

✍ Scribed by Edmund H. Schwartzel Jr.; S. Bachman; Robert A. Levine

• Book ID: 102982631
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 663 KB
• Volume: 78
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90100-2

✦ Synopsis

Published values of adenosine 3',5'-monophosphate (CAMP) and guanosine 3',5'-monophosphate (cCiMP) in gastrointestinal tissues and fluids have varied depending upon extraction and purification methods and assay procedures. Cyclic nucleotide levels in a variety of gastrointestinal tissues and fluids from several animal species were measured by the Gilman protein kinase assay for CAMP, and by a radioimmunoassay for CAMP and cGMP. The neutral alumina/Dowex-lformate column was the most accurate for the preparation of bile and gastric juice samples for the measurement of CAMP and cGMP, as verified by the use of column blanks, spiked samples, phosphodiesterase treatment, and serial sample dilution. Tissue samples gave consistent results regardless of the various column procedures employed.