Cyclic AMP level in relation to different conditions of sporulation rhythm regulation inLeptosphaeria michotii(West) Sacc
✍ Scribed by Simone Jerebzoff-Quintin; Stephan Jerebzoff
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 493 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
When Leptosphaeria michotii was grown in conditions that permitted a stable periodicity of sporulation (asparagine 6.6raM in darkness or asparagine 2.6raM in continuous white light), the level of intracellular cyclic AMP was lower and the activity of the cyclic AMP phosphodiesterase higher in contrast to the cultures with an instable periodicity.
With asparagine 6,6mM and in darkness, theophylline (1 raM) increased the intracellular cyclic AMP level whereas caffeine (1 mM) had no effect. Yheophylline (0.01 and 0.1 mM) or caffeine (0.01 -1 raM) provoked a rhythm instability under these conditions. Isoproterenol (1 raM) increased the cyclic AMP level. Nevertheless, the instable rhythm observed in control fed with asparagine 2.6 mM in darkness, was partially stabilized with isoproterenol 0.01 gM or 0.01-1raM. Exogenous cyclic AMP (0.01-1 raM) provoked a complete regulation of the rhythm with asparagine 2.6raM and a shortening of the stable period (from 27 to 21 h) when the fungus was grown on asparagine 6.6 raM.
These results underlined the fact that Lep tosphaeria rhythm regulation was not dependent on the cyclic AMP level only.