Cyclic AMP-induced inhibition of collagen lattice contraction by fibroblasts may be attenuated by both cyclic AMP dependent and independent mechanisms
✍ Scribed by Jan-Kan Chen; Shing-Rong Li; Ray Jui-Fung Tsai
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 604 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Taiwan, Republic of China
The contraction of collagen lattices made with foreskin fibroblasts in medium containing 1 % fetal bovine serum was inhibited by intracellular cyclic AMPraising drugs including cholera toxin (CT), forskolin, and dibutyryl-CAMP. The inhibition by CT was attenuated by insulin, acidic fibroblast growth factor (aFGF), and transforming growth factor-P (TGF-P). All three peptide factors have previously been reported to promote collagen lattice contraction by arterial smooth muscle cells and/or fibroblasts. Incubation of cells suspended in collagen gels with CT and forskolin resulted in a transient rise of the intracellular cyclic AMP levels, which peaked at 2 hr and 30 min, respectively, after drug exposure. Cholera toxin-induced intracellular cyclic AMP increase was attenuated by TGF-P, but not by aFCF and insulin, when added simultaneously. Thus, TGF-P may attenuate CT's inhibition on collagen lattice contraction by attenuating CTinduced intracellular cyclic AMP increse, whereas the attenuation by insulin and aFGF on the inhibition of lattice contraction may be mediated by a cyclic AMPindependent mechanism. o 1993 Wiley-Liss. Inc.