Cyclic AMP does not modulate hexose uptake of serum-deprived BHK-21 fibroblasts

✍ Scribed by Victor R. Lavis; Roxann Davenport; Paul Pontier; W. Joseph Thompson; Samuel J. Strada

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 327 KB
• Volume: 112
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041120310

✦ Synopsis

Abstract

We have studied the uptakes of the glucose analogs, 2‐deoxyglucose (2DG), and 3‐O‐methylglucose (3OMG) in serum‐deprived BHK‐21 fibroblasts. Incubation for 4 hours with 0.25 mM 1‐methyl‐3‐isobutylxanthine (MIX) increased cellular cyclic AMP 3 to 15 fold, without affecting the initial rates of uptake of these sugars. Incubation with 100 nM insulin, doubled hexose uptake without affecting cyclic AMP. Insulin did not modify the cyclic AMP response to MIX; nor did MIX alter the hexose uptake response to insulin. There was no effect of 0.1 mM L‐isoproterenol (ISO) or 0.1 mM prostaglandin E~1~ (PGE~1~) on cyclic AMP. PGE~1~ slightly stimulated uptake of 2DG but not 3OMG; ISO did not affect hexose uptake. We conclude that cyclic AMP does not directly regulate hexose uptake in these cells.