The dissociation of cyclic AMP-dependent protein kinase to give two catalytic subunits results in data for the binding reaction that cannot be interpreted by the method of Scatchard. Linear plots that allow determination of total receptor capacity and equilibrium association constant are described.
Cyclic AMP-dependent protein kinase: Purification of the holoenzyme by affinity chromatography
β Scribed by Susan S. Taylor; Chi-Yu Lee; Leslie Swain; Patrick H. Stafford
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 408 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0003-2697
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