✦ LIBER ✦

Cyanogen bromide cleavage of methionin residues as a control method for enkephalin immunocytochemistry

✍ Scribed by G. Jirikowski

Book ID: 104786766
Publisher: Springer
Year: 1985
Tongue: English
Weight: 788 KB
Volume: 83
Category: Article
ISSN: 1432-119X
DOI: 10.1007/bf00495307

No coin nor oath required. For personal study only.

✦ Synopsis

Serial semithin sections of rat neurohypophysis were immunostained with 2 antibodies to enkephalins using the peroxidase antiperoxidase method. One of the antibodies (R133) recognizes both met-and leu-enkephalin whereas the other (R26) reacts with met-enkephalin only. After cyanogen bromide pretreatment of the sections the antibody R 133 stained only a subpopulation of nerve endings that were distinct from those stained with the latter antibody. R 26-(met-enkephalin-like) immunoreactivity was totally abolished by cyanogen bromide pretreatment. This preincubation method which selectively interferes with the staining of met-enkephalin terminals may help to discriminate the two enkephalins in immunocytochemical preparations.

📜 SIMILAR VOLUMES

Oxidation of methionine to methionine su

Oxidation of methionine to methionine sulfoxide as a side reaction of cyanogen bromide cleavage

✍ Regula Joppich-Kuhn; Jeffrey A. Corkill; Roger W. Giese 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 378 KB

In addition to cleaving the peptide bond in N-pentafluorobenzoyl-methionyl-glycine, cyanogen bromide can also oxidize this peptide to the corresponding sulfoxide, particularly at a higher pH. The yields of this sulfoxide are 1, 8, 64, and 97% when the reaction conditions are 70% formic acid, 0.1 M h