Cyanobacterial toxins detected by Thamnotoxkit (a double blind experiment)

✍ Scribed by Andrea Kozma Törökné; Erzsébet László; Ingrid Chorus; Kaarina Sivonen; Francisco A. R. Barbosa

• Book ID: 101392841
• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 63 KB
• Volume: 15
• Category: Article
• ISSN: 1520-4081
• DOI: 10.1002/1522-7278(2000)15:5<549::aid-tox27>3.0.co;2-z

✦ Synopsis

The Thamnotoxkit F TM was evaluated for detecting cyanobacterial toxins compared with the rat hepatocyte test and mouse test. This test kit is a 24-h bioassay using larvae of the freshwater anostracan crustacean Thamnocephalus platyurus hatched from cysts. Thirteen freeze-dried cyanobacterial samples from freshwaters of Hungary, Germany, and Brazil, and strains from the culture collection of the University of Helsinki were tested and analyzed for microcystins and anatoxin a by high performance liquid chromatography and in some cases, the enzyme-linked immunosorbent assay test. The samples were examined by a double blind approach. The Thamnotoxkit test reacts to pure microcystins, neurotoxins, and crude extracts of cyanobacteria. It can be concluded that the Thamnotox test is an alternative simple, cost-effective method that may replace the mouse bioassay used previously for determination of cyanobacterial toxicity.