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Current Protocols in Toxicology || Assay of Tissue Activity of Nitric Oxide Synthase

โœ Scribed by Maines, Mahin D.; Costa, Lucio G.; Hodgson, Ernest; Reed, Donald J.; Sipes, I. Glenn

Book ID
120005943
Publisher
Wiley (John Wiley & Sons)
Year
1999
Tongue
English
Weight
155 KB
Volume
00
Category
Article
ISBN-13
9780471140856
ISSN
1934-9254

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โœ Roberto R. Giraldez; Jay L. Zweier ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 124 KB

While the L-arginine conversion assay has been utilized to measure nitric oxide synthase (NOS) activity in isolated enzyme and pure cell preparations, this method often fails to provide accurate measurements in whole tissues. Biological tissues contain variable amounts of unlabeled substrate and enz