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Current Protocols in Cytometry || Zinc Fixation for Flow Cytometry Analysis of Intracellular and Surface Epitopes, DNA Content, and Cell Proliferation

✍ Scribed by Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dobrucki, Jurek; Hyun, William C.; Nolan, John P.; Orfao, Alberto; Rabinovitch, Peter S.

Book ID
121475641
Publisher
John Wiley & Sons, Inc.
Year
2011
Weight
180 KB
Category
Article
ISBN-13
9780471142959

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Zinc fixation preserves flow cytometry s
Zinc fixation preserves flow cytometry scatter and fluorescence parameters and allows simultaneous analysis of DNA content and synthesis, and intracellular and surface epitopes
✍ Uffe Birk Jensen; David M. Owens; SΓΈren Pedersen; Rikke Christensen πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons 🌐 English βš– 881 KB

## Abstract Zinc salt‐based fixation (ZBF) has proved advantageous in histochemical analyses conducted on intact tissues but has not been exploited in flow cytometry procedures that focus on quantitative analysis of individual cells. Here, we show that ZBF performs equally well to paraformaldehyde