Current Protocols in Cytometry || Dual-Pulse Labeling Using 5-Ethynyl-2′-Deoxyuridine (EdU) and 5-Bromo-2′-Deoxyuridine (BrdU) in Flow Cytometry

Cell type specific applicability of 5-et

Cell type specific applicability of 5-ethynyl-2′-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry

✍ Simone Diermeier-Daucher; Scott T. Clarke; Dani Hill; Arabel Vollmann-Zwerenz; J 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 919 KB

## Abstract Using the nucleoside analogue EdU (5‐ethynyl‐2′‐deoxyuridine) for thymidine substitution instead of BrdU (5‐bromo‐2′‐deoxyuridine) in cell proliferation assays has recently been proposed. However, the effect of EdU on cell viability, DNA synthesis, and cell cycle progression and consequ