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Current Protocols in Cell Biology || Measuring Protein Mobility by Photobleaching GFP Chimeras in Living Cells

✍ Scribed by Bonifacino, Juan S.; Dasso, Mary; Harford, Joe B.; Lippincott-Schwartz, Jennifer; Yamada, Kenneth M.

Book ID
118142187
Publisher
Wiley (John Wiley & Sons)
Year
2003
Tongue
English
Weight
548 KB
Volume
19
Category
Article
ISBN-13
9780471143031
ISSN
1934-2500

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## Abstract The use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin‐receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin recep