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Current Protocols in Cell Biology || In Vitro Analysis of Yeast Mitochondrial Protein Import

โœ Scribed by Bonifacino, Juan S.; Dasso, Mary; Harford, Joe B.; Lippincott-Schwartz, Jennifer; Yamada, Kenneth M.

Book ID
121655592
Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
154 KB
Volume
34
Category
Article
ISBN-13
9780471143031
ISSN
1934-2500

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Functional analysis of mitochondrial pro
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โœ Scott M. Glaser; Cynthia E. Trueblood; Lori K. Dircks; Robert O. Poyton; Michael ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 866 KB

In order to facilitate studies on protein localization to and sorting within yeast mitochondria, we have designed an experimental system that utilizes a new vector and a functional assay. The vector, which we call an LPS plasmid (for leader peptide substitution), employs a yeast COX% gene (the struc