A QB RNA segment was produced i n vitro by means of a synchronized minus-strand-directed synthesis using purified QP replicase, a reaction known to yield infectious QB RNA. Arguments are presented that a 5.5s product obtained by incubation for 30 seconds at 2 0 ยฐC corresponds to the 5'-terminal regi
Current approaches to analysis of the nucleotide sequence of DNA
โ Scribed by Sherman M. Weissman
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 953 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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After reviewing approaches to the nucleotide correlation of DNA sequences the preferential mode analysis method is emphasized and discussed in detail. The preferred modes and poor modes in coding regions, as well as in introns, 5'-caps and 3'-tails are found through the statistical analysis of seque
The vibrational spectra of four genomic and two synthetic DNAs, encompassing a wide range in base composition [poly(dA-dT) 72% G ฯฉ C; poly(dG-dC)โ poly(dG-dC), 100% G ฯฉ C] (dA: deoxyadenosine; dG: deoxyguanosine; dC: deoxycytidine; dT: thymidine), have been analyzed using Raman difference methods of