Abscisic acid and precursors, from plant tissues, assay, 289, 251 Acetylcholinesterase distribution in brain, histochemical measurement using digital scanning densitometry, 296, 18 N-Acetyl-L-cysteine reduced, protein-unbound, and total concentrations in plasma, postcolumn ligand substitution HPLC assay, 290, 251 N-Acetylglucosamine O-linked, murine monoclonal antibody specific to, characterization, 293, 169 N-Acetyl-D-glucosamine reducing ends of polysaccharides, assay in presence of proteins, 293, 53 N-Acetylglucosaminyltransferase V inhibitors of, in mixtures, screening by frontal affinity chromatography coupled to mass spectrometry, 299, 173 N-Acetyltransferase 2 genotype determination with single nucleotide polymorphism-specific PCR primers and fluorogenic probes (human), 288, 106 ␣ 1 -Acid glycoprotein fucosylation, determination with lectin immunosensor, 288, 216 Acidic fibroblast growth factor interaction with heparin, analysis using evanescent wave biosensor with regioselectively desulfated heparins as ligands, 295, 203 Acridine orange donor in long-wavelength luminophores with long lifetime and high quantum yield, 288, 62 -Actin gene comparison to 18S rRNA as internal standard for mRNA assay in human melanoma cell subpopulations, 295, 17 Actinomycin D apoptosis induced by, characterization by capillary zone electrophoresis, 297, 10 Active sites thrombin and other serine proteases, labeling by biotin derivatives of D-Phe-Pro-Arg-CH 2 Cl, 296, 254 Acylating agents inactivation of antinuclear antibodies, protective effect of negatively charged polymers, 292, 245 Acyl-coenzyme A esters formed by valproyl-CoA -oxidation, isolation and intramitochondrial levels, 290, 60 Adenosine deaminase CP-I subunit, purification with electroosmotic medium pump system for preparative disk gel electrophoresis, 288, 168 Adenoviruses recombinant analysis with integrated microfluidic chip-based system, 291, 262 stabilization, analysis by site-directed mutagenesis of asparagine in hexon protein, 297, 1 Adipogenic differentiation high-capacity screen for, 293, 124 Adipose tissue brown, mRNA levels, assay using nonradioisotopic detection of PCR products, 293, 152 ADP-ribosyl cyclase reverse-phase HPLC assay, 299, 218 Adsorption DNA on mica for atomic force microscopy, control by chemical modification of mica surface, 290, 232 mechanistically concerted processes, analysis, optical biosensors for, review, 288, 109 Adsorptive stripping voltammetry determination of tungsten and molybdenum in proteins, 297, 71 Aequorin epitope-aequorin conjugate recognition in immunoassays for complex proteins, 294, 132 Affinity adsorption cystatin-based, in isolation and analysis of papain-like cysteine proteinases from tissue extracts, 289, 231 Affinity chromatography frontal, coupled to mass spectrometry, for screening mixtures of enzyme inhibitors, 299, 173 L-lactate dehydrogenase with polyhistidine tag: analysis of activity loss, 295, 257 monoclonal antibodies, peptide mimetic ligands for, generation and refinement, 296, 9 prediction of affinity and kinetics in biomolecular interactions, 291, 102 with Ricinus communis affinity adsorbents prepared with polymeric supports, 295, 66 transcription factors, review, 290, 147 Agarose gel slices pellet pestle homogenization at 45°C for DNA extraction, 296, 162 Agglutinins Ricinus communis, affinity adsorbents, preparation and evaluation with polymeric supports, 295, 66 wheat germ, interactions with immobilized metal ions, analysis using isotherm models, 288, 126 AKT serine/threonine kinase competitive fluorescence polarization assay using product-specific anti-phospho-serine antibody, 299, 45 Alamethecin visual microwell plate assay, 293, 258 Alanine aminotransferase conformationally restricted ␣-ketoglutarate analogues as substrates of, evaluation, 298, 265 Alcohol dehydrogenase  subunit, human liver diacetyl reductase DAR-2 identification as, 293, 157 Aldehyde reductase human liver diacetyl reductase DAR-7 identification as, 293, 157 286