Absorbance capacity oxygen radicals, semiautomatic determination, device for, 287, 226 AB toxins plant, calibration of colorimetric protein assay for, 284, 418 Acetic acid and methanol, cell fixation with, effect on chromatin topography, 282, 29 N-Acetyl-D-Ala-D-Ala in affinity capillary electrophoresis for estimation of receptorligand interactions, 280, 209 N-Acetylaspartate in cerebral tissue extracts, detection by ion-pairing HPLC, 277, 104 Acetylcholinesterase active sites and turnover, estimation in insects, 285, 76 N-Acetylglutamate in cerebral tissue extracts, detection by ion-pairing HPLC, 277, 104 N-Acetylneuraminic acid in gangliosides, assay by neuraminidase hydrolysis and fluorometric HPLC, 281, 193 N-Acetyltransferase NAT1 and NAT2 genes, polymorphism in, simultaneous determination by reverse line blot hybridization, 285, 156 Acidic oligosaccharides electrophoretic analysis, thematic review, 283, 136 Acrylic acid polyethylene-coacrylic acid film without molecular labels or surface-fouling agents, SLT gene detection on, 282, 218 Actin filaments solution viscosity, modification by aluminum, measurement by optical displacement microviscometry, 277, 1 Activated protein C determination with galactosidase protease-activated receptor, 277, 33 Acute lymphoblastic leukemia therapy with L-asparaginase, monitoring by fluorometric assay, 280, 42 Acylethanolamides bioactive, assay in rat plasma by electrospray MS, 280, 87 S-Adenosyl-L-homocysteine in nonradioisotopic analysis of isoaspartate in peptides and proteins, 282, 227 Aequorin luminescence, Ca 2ϩ measurements effects of halothane, enflurane, and isoflurane, 284, 60 high-throughput glow-type assay of receptor-mediated intracellular changes, 286, 231 recombinant, in bioluminescence detection of proteolytic bond cleavage, 281, 87 Affinity biosensor for avidin, using double functionalized dendrimer monolayer on gold electrode, 282, 121 Affinity capillary electrophoresis polyclonal antihapten antibody characterization, 284, 422 two-marker system in, for estimation of receptor-ligand interactions, 280, 209 Affinity capture DNA at femtomolar concentrations with peptide nucleic acid probes, 283, 241 Affinity chromatography DNA-based, for transcription factors, temperature dependence, 279, 209 mitochondrial cytochrome c binding to bacterial photosynthetic reaction center in liposome membranes, 280, 94 receptor analog-based, using binding of avian ovomucoid to Shigalike toxin type 1, 281, 202 Affinity ligands biotinylated phosphatidylinositol 3,4,5-trisphosphate as, 280, 301 Agarose chemiluminescent electromobility shift assays based on, use of biotin-labeled nucleic acids in, 277, 254 Agrobacterium tumefaciens large-sized genomic DNA, isolation within one hour, scalable protocol for, 278, 86 Akt1 assay using phosphopeptide detection by fluorescence polarization in presence of cationic polyamino acids, 278, 206 D-Alanine fluorescent coupled enzyme assay, application to penicillin-binding protein and vancomycin activity assays, 287, 196 Alcian blue in assay and isolation of anionic glycoconjugates from cell cultures, 285, 211 Aldobiuronic acid 4-nitrophenyl glycoside of, as chromogenic substrate for -xylosidase-coupled assay of ␣-glucuronidase, 286, 289 Alkaline phosphatase in assay of blocking effect of Tween 20 and bovine serum albumin in ELISA microwells, 282, 232 in homogeneous cell-based assay of N-linked carbohydrate processing inhibitors, 280, 137 immobilization on solid supports with retention of activity, 280, 178 placental, in reporter gene assay of G-protein-coupled receptors expressed in HEK293 EBNA cell suspension culture, 284, 316 in pyrosequencing on double-stranded DNA, 282, 186 AlkA protein in vivo dimethyl sulfate footprinting with, analysis of DNA-protein interactions at mouse H19 gene promoter in primary embryo fibroblasts, 283, 112 Alkylation thiols below neutral pH, 286, 309 O 6 -Alkylguanine-DNA alkyltransferase real-time fluorescence assay, 281, 216