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Cultured mammary carcinoma cells contain gonadotropin-releasing hormone-like immunoreactivity, gnrh binding sites and chorionic gonadotropin

✍ Scribed by R. Bützow; I. Huhtaniemi; R. Clayton; T. Wahlström; L. C. Andersson; M. Seppälä


Publisher
John Wiley and Sons
Year
1987
Tongue
French
Weight
569 KB
Volume
39
Category
Article
ISSN
0020-7136

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✦ Synopsis


lmmunoperoxidase staining and radioimmunoassay were used to identify gonadotropin-releasing hormone (GnRH) and chorionic gonadotropin (hCG) in 4 mammary carcinoma cell lines. GnRH-like immunoreactivity was found in all cell lines; 2 lines also contained hCG. Binding of a GnRH agonist was demonstrated by radioreceptor assay in cultured mammary carcinoma cells. In cell column perfusion of the same cells, exogenous GnRH did not stimulate release of hCG.