Cultured human leukemic non-T/non-B lymphoblasts and their stimulating capacity in “one-way”mixed lymphocyte reaction. Suggestive evidence for early T-cell or B-cell precursors
✍ Scribed by Tin Han; Barbara Dadey; Jun Minowada
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 463 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
✦ Synopsis
It is now well recognized that a large proportion of cases with acute lymphoblastic leukemia are classified as non-T/non-B neoplastic disease. The origin of leukemic non-Thon-B cells is at present not known. It has been shown that fresh or cultured leukemic T lymphoblasts exert no stimulating capacity while leukemic B lymphoblasts exert a strong stimulation in "one-way" mixed lymphocyte reaction. It has also been shown that fresh leukemic cells from some patients with non-T/non-B acute lymphoblastic leukemia possess a strong stimulation while leukemic cells from other patients with this disease possess no stimulation on allogeneic lymphocytes. The present study shows that cultured leukemic lymphoblasts from 3 non-Tlnon-B cell lines (NALL-l, NALM-6 and NALM-16) consistently exert a strong stimulation on allogeneic lymphocytes. On the other hand, cultured leukemic lymphoblasts from 2 non-T/non-B cell lines (REH and KM-3) consistently fail to stimulate in "one-way" mixed lymphocyte reaction. Our data clearly support the speculation that leukemic non-Thon-B cells which possess the stimulating capacity may represent less differentiated leukemic B lymphoid cells (pre-B cells) and leukemic non-T/ non-B cells which possess no stimulating capacity may represent less differentiated leukemic T lymphoid cells (pre-T cells).
Cancer 44:136-140, 1979. INCE THE RECENT INTRODUCTION of surface S marker studies of leukemic cells, it was found that the T-cell acute lymphoblastic leukemia (ALL) represents 15-25%, the B-cell ALL represents less than 5% and the non-Tlnon-B ALL represents more than 70% of cases.1,2*4,13,14,25 The origin of leukemic non-Tlnon-B lymphoblasts is at present not known. Recently reported antigen marker studies of leukemic non-T/non-B cells are conflicting; some investigators consider non-Tl non-B ALL as either B stem cell neoplastic disease3 or T-cell lineage leukemia,24 while others consider non-T/non-B ALL as both T stem cell and B stem cell leukemia." It has been demonstrated that fresh or