Cultured brain endothelium inhibits the cytocidal action of natural killer cells on glioma

✍ Scribed by Jack M. Rozentall; Grazyna M. Kaminska; Marek J. Kaminski

• Publisher: Springer US
• Year: 1989
• Tongue: English
• Weight: 360 KB
• Volume: 7
• Category: Article
• ISSN: 0167-594X
• DOI: 10.1007/bf00149380

✦ Synopsis

The killing of GL26 and YAC-1 cells by natural killer cells (NKC) is reduced in the presence of a monolayer of endothelial cells. This reduction in cytotoxicity correlates with the degree of adhesion between the tumor cells and the endothelial monolayers. The cytotoxicity of NKC toward glioma was 1007o when carried out on plastic, but a monolayer of endothelium derived from brain inhibited the cytotoxicity by about 90%. Endothelium from thoracic duct and lung also inhibited cytotoxicity by about 90%, endothelium from aorta inhibited by 55% and that from ovary by only 45%. Cytotoxicity of NKC toward YAC-1 (a control NK target) was 40% on plastic, but a monolayer of endothelium from thoracic duct inhibited the cytotoxicity by 75%. Endothelium from brain and lung inhibited cytotoxicity by about 60%, aorta by 50%, and ovary by 40°/0. Interactions between tumor cells and the host-organ microvascular endothelium appear to protect neoplastic cells from natural surveillance mechanisms and may play a role in the formation of metastatic tumor deposits.