✦ LIBER ✦

Cultured astrocytes express functional receptors for galanin

✍ Scribed by Josef Priller; Carola A. Haas; Martin Reddington; Georg W. Kreutzberg

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 102 KB
Volume: 24
Category: Article
ISSN: 0894-1491
DOI: 10.1002/(sici)1098-1136(199811)24:3<323::aid-glia6>3.0.co;2-2

No coin nor oath required. For personal study only.

✦ Synopsis

The neuropeptides galanin and calcitonin gene-related peptide (CGRP) are strongly up-regulated in motoneurons following axotomy. Earlier reports have suggested that peptides might be released from injured neurons to recruit surrounding glia. In this study, the effects of galanin and CGRP on cultured rat astrocytes were investigated using the expression of immediate early genes as a model for receptormediated transcriptional activation. Galanin was found to induce c-fos, junB, and Tis11 mRNA in cultured astrocytes, providing evidence for the presence of functional galanin receptors on neuroglial cells. In contrast, CGRP only led to the induction of c-fos and junB mRNA. Cholecystokinin (CCK-8) and substance P, which are also up-regulated in select motoneuron populations following axotomy, fail to induce immediate early genes in astrocytes, indicating specificity of neuropeptides in their ability to stimulate glial cells. The differential induction of immediate early gene expression by galanin and CGRP in astrocytes points to differences in intracellular signal transduction mechanisms. Whereas CGRP was found to stimulate the accumulation of cyclic AMP by 10-to 20-fold, galanin had no effect on basal cyclic AMP content. The effect of CGRP on cyclic AMP accumulation was completely reversed by the CGRP receptor antagonist, CGRP . These results suggest roles for galanin and CGRP in the transcriptional activation of astrocytes.

📜 SIMILAR VOLUMES

Cultured astrocytes express toll-like re

Cultured astrocytes express toll-like receptors for bacterial products

✍ Christal C. Bowman; Amy Rasley; Susanne L. Tranguch; Ian Marriott 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 368 KB

## Abstract It has become apparent that astrocytes may be important contributors to inflammatory immune responses within the brain in response to microbial challenges. To date, the mechanisms that underlie activation of this major glial cell type by such challenges have not been investigated. In th

AMPA receptor protein expression and fun

AMPA receptor protein expression and function in astrocytes cultured from hippocampus

✍ Dali Fan; Sonja Y. Grooms; Ricardo C. Araneda; Anne B. Johnson; Kostantin Dobren 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 609 KB

Glutamate receptors guide the proliferation, migration, and differentiation of glial cells. Here, we characterize AMPA (␣-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid) and NMDA receptor protein expression and function and mRNA expression in hippocampal glial cultures. By immunocytochemistry,

Nucleoside transporter expression and fu

Nucleoside transporter expression and function in cultured mouse astrocytes

✍ Liang Peng; Rong Huang; Albert C.H. Yu; King Y. Fung; Michel P. Rathbone; Leif H 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 327 KB

## Abstract Uptake of purine and pyrimidine nucleosides in astrocytes is important for several reasons: (1) uptake of nucleosides contributes to nucleic acid synthesis; (2) astrocytes synthesize AMP, ADP, and ATP from adenosine and GTP from guanosine; and (3) adenosine and guanosine function as neu

Developmental expression of rat brain mi

Developmental expression of rat brain mitogens for cultured astrocytes

✍ Richard S. Morrison; Russell P. Saneto; Jean de Vellis 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 421 KB 👁 1 views

## Abstract Extracts prepared from prenatal, neonatal, and adult rat brain were examined for the presence of astrocyte mitogens. Extracts were characterized by molecular weight separation on Sephadex G‐100. Two major peaks of mitogenic activity, at 80K and 23K daltons, were identified in the adult

Kainate/AMPA receptors expressed on huma

Kainate/AMPA receptors expressed on human fetal astrocytes in long-term culture

✍ Keith Cauley; Valery Kukekov; David Young 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 245 KB

Long-term cultivation of primary human fetal brain cells has yielded a homogeneous population of glial progenitors of extended life span. These human astrocyte precursor (HAP-1) cells have been in culture for greater than 1 year, are diploid, and do not form colonies in soft agar. The culture was es

Sub-population of cultured hippocampal a

Sub-population of cultured hippocampal astrocytes expresses neuropeptide Y Y1 receptors

✍ Jacques-André St-Pierre; Dominique Nouel; Yvan Dumont; Alain Beaudet; Rémi Quiri 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 800 KB

The expression and pharmacological characterization of neuropeptide Y (NPY) receptors of the Y 1 subtype on cultured hippocampal neurons was reported using radioreceptor assays and immunohistochemical approaches (St-Pierre et al., 1998). The present study aimed to establish the presence of NPY Y 1 r