Culture of human epidermal Langerhans cells in a skin equivalent
โ Scribed by Fransson; Heffler; Tengvall Linder; Scheynius
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 423 KB
- Volume
- 139
- Category
- Article
- ISSN
- 0007-0963
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Langerhans cells (LCs) are dendritic cells present in many epithelia. They are of mesenchymal origin, as shown by the absence of desmosomes and tonofilaments and the presence of cytoplasmic vimentin [3]. It has been shown that LCs assume a key role in the cutaneous immune system [14] and, in humans,
Kinetic data on the labeling index (LI), DNA synthesis time (ts), and potential doubling time (tpot) of epidermic cells in relation to sex, age, and site were obtained by in vitro incubation of skin cylinders from 45 human cadavers with DNA precursors 3H- and 14C-thymidine. In a first study on parts
There is controversy about whether Cyclosporin A (CsA) affects antigen-presenting cell function. Within the skin, Langerhans cells (LC) are very potent antigenpresenting cells. We investigated the effect of CsA on alloantigen presentation by human LC using the in vitro mixed skin-cell lymphocyte rea