## Abstract __Escherichia coli__, expressing recombinant green fluorescent protein (GFP), was subjected to dissolved oxygen tension (DOT) oscillations in a two‐compartment system for simulating gradients that can occur in large‐scale bioreactors. Cells were continuously circulated between the anaer
✦ LIBER ✦
Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: Metabolic response and production of recombinant protein
✍ Scribed by Edgar A. Sandoval-Basurto; Guillermo Gosset; Francisco Bolívar; Octavio T. Ramírez
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 338 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0006-3592
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## Abstract At the laboratory scale, sudden step increases from 30 to 42°C can be readily accomplished when expressing heterologous proteins in heat‐inducible systems. However, for large scale‐cultures only slow ramp‐type increases in temperature are possible due to heat transfer limitations, where