Culture Medium Composition for Optimal Thallus Regeneration in the Red Alga Asparagopsis armata Harvey (Rhodophyta, Bonnemaisoniaceae)

✍ Scribed by Haslin, C.; Pellegrini, M.

• Book ID: 118192028
• Publisher: Walter de Gruyter GmbH & Co. KG
• Year: 2001
• Tongue: English
• Weight: 168 KB
• Volume: 44
• Category: Article
• ISSN: 0006-8055
• DOI: 10.1515/bot.2001.004

✦ Synopsis

The formation and the growth of regenerated fronds from cultivated barbs of Asparagopsis armata were investigated according to an experimental design methodology. A maximal ratio of 3.3 mm regeneration/mm barb was reached for two media constituted of seawater complemented with iron sulfate, EDTA and potassium bromide. The first optimal medium was composed of [FeSO 4 7H 2 O] ϭ 0.20 mg L Ϫ1 , [Na 2 EDTA2H 2 O] ϭ 2.9 mg L Ϫ1 , [KBr] ϭ 12.0 mg L Ϫ1 and the second one of [FeSO 4 7H 2 O] ϭ 0.2 mg L Ϫ1 , [Na 2 EDTA2H 2 O] ϭ 4.6 mg L Ϫ1 , [KBr] ϭ 28.7 mg L Ϫ1 . Barbs excised from adult thalli were placed for 4 weeks in seawater enriched with several chemical substances at different concentrations. The experimental procedure involved: 1) the screening of the influencing compounds with an Hadamard design and 2) the investigation of their optimal concentrations according to a Doehlert design of response surface methodology.