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Cultivation of recombinant E. coli and production of fusion protein in 60-l bubble column and airlift tower loop reactors

✍ Scribed by H.A. Kracke-Helm; U. Rinas; B. Hitzmann; K. Schügerl

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
835 KB
Volume
13
Category
Article
ISSN
0141-0229

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Fed-batch cultivation of recombinant escherichia coli JM103 and production of the fusion protein SPA::EcoRI in a 60-L working volume airlift tower loop reactor
✍ Lutz Brandes; Xiaoan Wu; Jens Bode; Jong II Rhee; Karl Schügerl 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 852 KB

EcoRI fusion protein was produced by Escherichia coli JM103 carrying the multicopy expression plasmid pMTC48, the multicopy repressor plasmid pRK248, and the multicopy protection plasmid pEcoR4 in a 60-L working volume airlift tower loop reactor on M9 minimal medium with glucose. Cell mass concentra