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Ctyoplasmic DNA: Separation into two components with detergents

โœ Scribed by Walter C. Schneider

Book ID
119405884
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
212 KB
Volume
62
Category
Article
ISSN
0006-291X

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A general procedure for purifying and efficiently separating four types of DNA from filamentous fungi has been developed. The protocol involves (i) disruption of mycelial cells by blending in liquid nitrogen followed by suspension of cell contents in buffer containing high concentrations of protease