Crystallization, X-ray diffraction analysis and SIRAS phasing of human α- L -iduronidase
✍ Scribed by Maita, Nobuo; Taniguchi, Hisaaki; Sakuraba, Hitoshi
- Book ID
- 119771611
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 835 KB
- Volume
- 68
- Category
- Article
- ISSN
- 1744-3091
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi
## Abstract Nonglycosylated α‐amylase, a major component of human parotid saliva, has been crystallized by the vapor diffusion technique using 2‐methyl‐2,4‐pentanediol as the precipitant in the presence of CaCl~2~ at pH 9.0. The crystals are orthorhombic, space group __P__2~1~2~1~2~1~ with unit cel