Crystallization chaperone strategies for membrane proteins
โ Scribed by Raquel L. Lieberman; Jeffrey A. Culver; Kevin C. Entzminger; Jennifer C. Pai; Jennifer A. Maynard
- Book ID
- 116987313
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 944 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1046-2023
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bod
Viral membrane proteins extracted from Sendai virions with the non-ionic detergents decylpolyethyleneglycol-300 and Triton X-100 were used as a model mixture of hydrophobic membrane proteins. The detergent extract contained the fusion protein (F) and the tetrameric and dimeric forms of the hemagglut