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Crystallization and preliminary x-ray studies of I-CreI: a group I intron-encoded endonuclease from C. reinhardtii

โœ Scribed by Kathryn M. Stephens; Raymond J. Monnat Jr.; Patrick J. Heath; Barry L. Stoddard

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
78 KB
Volume
28
Category
Article
ISSN
0887-3585

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โœฆ Synopsis

Group I intron endonuclease I-CreI is encoded by an open reading frame contained within a self-splicing intron in the Chlamydomonas reinhardtii chloroplast 23S rRNA gene. I-CreI initiates the lateral transfer or homing of this intron by specifically recognizing and cleaving a pseudopalindromic 19-24 bp homing site in chloroplast 23S rRNA genes that lack the intron. The gene encoding this enzyme has been subcloned, and the protein product has been purified and crystallized. The crystals belong to space group P321, with unit cell dimensions a 5 b 5 78.2 ร…, c 5 67.4 ร…. The crystal unit cell is consistent with an asymmetric unit consisting of the enzyme monomer. The specific volume of this unit cell is 3.3 ร… 3 /Da. The crystals diffract to at least 3.0 ร… resolution after flash-cooling, when using a rotating anode x-ray source and an RAXIS image plate detector.

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