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Crystallization and Preliminary X-ray Diffraction Studies on ADP-ribosylation Factor 1

✍ Scribed by Samantha Greasley; Harren Jhoti; Amanda C. Fensome; Shamshad Cockcroft; Geraint M.H. Thomas; Ben Bax


Book ID
115626971
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
143 KB
Volume
244
Category
Article
ISSN
0022-2836

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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi