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Crystallization and preliminary X-ray diffraction studies of the spinach-chloroplast thioredoxin f

✍ Scribed by Jean-Claude Génovésio-Taverne; Yvonne Jetzer; Ursula Sauder; Erhard Hohenester; Christelle Hughet; Johan N. Jansonius; Laura Gardet-Salvi; Peter Schürmann


Book ID
115985624
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
264 KB
Volume
222
Category
Article
ISSN
0022-2836

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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi