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Crystallization and preliminary X-ray diffraction studies of the human major histocompatibility antigen HLA-B27

✍ Scribed by Joan C. Gorga; Dean R. Madden; John K. Prendergast; Dr. Don C. Wiley; Jack L. Strominger

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
392 KB
Volume
12
Category
Article
ISSN
0887-3585

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✦ Synopsis

Abstract

The class I major histocompatibility (MHC) antigen HLA‐B27 was purified by immunoaffinity chromatography from the homozygous human B lymphoblastoid cell line LG‐2. Detergent‐soluble HLA‐B27 was cleaved with the protease papain to remove the hydrophobic transmembrane region and the cytoplasmic tail. Crystals of the resulting water‐soluble extracellular fragments were obtained in hanging drops by the vapor‐diffusion method. The crystals are triclinic, space group __P__1, with unit cell dimensions a = 45.9 Å, b = 71.0 Å, c = 83.7 Å, α = 79.4°, β = 88.5°, γ = 89.9°, and diffract beyond 2.5 Å resolution.

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