✦ LIBER ✦
Crystallization and preliminary X-ray diffraction analysis of bovine seminal plasma PDC-109, a protein composed of two fibronectin type II domains
✍ Scribed by Antonio Romero; Paloma F. Varela; Edda Töpfer-Petersen; Juan J. Calvete
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 36 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0887-3585
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✦ Synopsis
PDC-109 is a 13 kDa glycoprotein and the major phosphorylcholine-and heparin-binding protein of bull seminal plasma. It is built by an acidic 23-residue N-terminal sequence followed by a tandem of fibronectin type II domains. Full-length PDC-109 was crystallized in complex with o-phosphorylcholine by vapor diffusion in sitting drops. Crystals grew to maximal size of 0.5 3 0.3 3 0.2 mm 3 , diffract x-rays beyond 2.6 Å resolution, and belong to space group P321 with unit cell dimensions a 5 b 5 93.6 Å, c 5 52.7 Å. Proteins 28