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Crystallization and Preliminary X-ray Analysis of Wild-type and K272A Mutant Glutamate 1-Semialdehyde Aminotransferase from Synechococcus

✍ Scribed by Michael Hennig; Bernhard Grimm; Margrit Jenny; Rita Müller; Johan N. Jansonius


Book ID
115626849
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
274 KB
Volume
242
Category
Article
ISSN
0022-2836

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Crystallization and preliminary X-ray an
✍ Markus Moser; Rita Müller; Johan N. Jansonius; Natalia Battchikova; Marianne Koi 📂 Article 📅 1996 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 321 KB

## Abstract Recombinant phosphoserine aminotransferase (EC 2.6.1.52) from __Bacillus circulans subsp. alkalophilus__ was crystallized at room temperature from 0.1 M sodium acetate buffer, pH 4.6, and 2% PEG 20000, using macroseeding techniques. The crystals diffract X‐rays to at least 2.0 Å nominal