Crystallization and Preliminary Crystallographic Analysis of Recombinant Xenopus laevis Cu, Zn Superoxide Dismutase b
β Scribed by K. Djinovic Carugo; C. Collyer; A. Coda; M.T. Carri; A. Battistoni; G. Bottaro; F. Polticelli; A. Desideri; M. Bolognesi
- Book ID
- 115573303
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 244 KB
- Volume
- 194
- Category
- Article
- ISSN
- 0006-291X
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## Abstract The Cu, Zn superoxide dismutase (Cu, Zn SOD) originally isolated from the periplasmic space of __Escherichia coli__ has been cloned and overexpressed in the __E. coli__ strain BMH 71/18. The protein has been purified as a single component of 17,000 Da, corresponding to one subunit of th
The crystallographic structure of bovine superoxide dismutase has been used as a template for the graphic reconstruction of the three-dimensional structures of the two Xenopus laevis variants (SchininΓ , M.E. et al. Arch. Biochem. Biophys. 272:507-515, 1989). In these models the structure-essential r