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Crystallization and preliminary cryogenic X-ray diffraction analyses of protein L-isoaspartyl O-methyltransferase from human fetal brain

✍ Scribed by Craig D. Smith; Joseph Barchue; Charles Mentel; Lawrence Delucas; Takuji Shirasawa; Debasish Chattopadhyay


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
39 KB
Volume
28
Category
Article
ISSN
0887-3585

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✦ Synopsis


Recombinant human fetal brain protein L-isoaspartyl O-methyltransferase, EC 2.1.1.77, was crystallized in PEG 8000 with adenosine homocysteine by a macroseeding technique. The space group was P2 1 with a 5 47.4 Å, b 5 53.9 Å, c 5 48.7 Å and b 5 116.4°f or cryofrozen crystals at 90 K. The crystals diffracted to 2.1 Å and have one molecule per asymmetric unit.