Crystallization and high-resolution X-ray diffraction data collection of an Asp49 PLA2 from Bothrops jararacussu venom both in the presence and absence of Ca2+ ions
✍ Scribed by Mário T. Murakami; Simone Michelan-Duarte; Adélia C.O. Cintra; Raghuvir K. Arni
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 106 KB
- Volume
- 1703
- Category
- Article
- ISSN
- 1570-9639
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✦ Synopsis
Snake venom PLA(2)s have been extensively studied due to their role in mediating and disrupting physiological processes such as coagulation, platelet aggregation and myotoxicity. The Ca(2+) ion bound to the putative calcium-binding loop is essential for hydrolytic activity. We report the crystallization in the presence and absence of Ca(2+) and X-ray diffraction data collection at 1.60 angstroms (with Ca(2+)) and 1.36 angstroms (without Ca(2+)) of an Asp49 PLA(2) from Bothrops jararacussu venom. The crystals belong to orthorhombic space group C222(1). Initial refinement and electron density analysis indicate significant conformational changes upon Ca(2+) binding.