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Crystalline phosphoglycerate kinase from human erythrocytes

✍ Scribed by Takashi Hashimoto; Haruhisa Yoshikawa

Book ID
115791507
Publisher
Elsevier Science
Year
1962
Weight
304 KB
Volume
65
Category
Article
ISSN
0006-3002

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Purification of human erythrocyte phosph
Purification of human erythrocyte phosphoglycerate kinase by dye ligand affinity chromatography
✍ Josiane Chen-Marotel; Yves Blouquit; Raymonde Rosa; Marie-Claude Calvin πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 906 KB

A new method for the purification of human erythrocyte phosphoglyceratekinase involving affinity chromatography on dye-ligand media (Red A), in the presence of 3-phosphoglycerate and ATP, is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophores