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Crystal Structure of Phospholipase C from Bacillus cereus Complexed with a Substrate Analog

✍ Scribed by Sissel Hansen; Edward Hough; L.Anders Svensson; Yue-Ling Wong; Stephen F. Martin


Book ID
115626224
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
850 KB
Volume
234
Category
Article
ISSN
0022-2836

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A continuous spectrophotometric assay fo
✍ William R. Snyder πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 558 KB

A thiophosphate analog of dioctanoylphosphatidylcholine has been used as the substrate in a continuous spectrophotometric assay for the Bacillus cereus phospholipase C. The reaction has been monitored at 412 nm using 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and at 324 nm using 4,4'-dithiopuridine