Cryopreservation of the promastigote form of Leishmania tropica var major at different cooling rates

Effect of cooling rate, cryoprotectant a

Effect of cooling rate, cryoprotectant and holding time at different transfer temperatures on the survival of cryopreserved cell suspension culture (Puccinellia distans(L.) Parl.)

✍ László E. Heszky; Zsolt Jekkel; Abdel-Hamid Ali 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 608 KB

Reflexed saltmarsh-grass suspension cultures produced by seed callus were frozen to the liquid nitrogen temperature. Cooling rates, cryoprotectants and holding times were taken as a function of transfer temperatures. The highest survival of cells (45%) was found at a freezing rate of I°C rain -I , w