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Cryopreservation of oil palm (Elaeis guineensisJacq.) somatic embryos involving a desiccation step

✍ Scribed by Dominique Dumet; Florent Engelmann; Nathalie Chabrillange; Yves Duval

Book ID: 104677066
Publisher: Springer
Year: 1993
Tongue: English
Weight: 550 KB
Volume: 12
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00237434

No coin nor oath required. For personal study only.

✦ Synopsis

The standard cryopreservation process previously developed for oil palm clones using shiny white, finger-like somatic embryos could be applied in some cases to standard cultures. Its efficiency was markedly improved by completing the 7-day pregrowth period on 0.75 M sucrose by an additional dehydration period carried out either by placing the embryos in the air current of the laminar flow cabinet or in an air tight box containing silica gel. This improved process was successfully applied to 7 different clones. It will facilitate the routine uof cryopreservation for oil palm cultures.

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