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Cryopreservation of monogonont rotifers

✍ Scribed by Charles E. King; H. Berkeley Bayne; Todd K. Cannon; Andrew E. King

Publisher: Springer
Year: 1983
Tongue: English
Weight: 268 KB
Volume: 104
Category: Article
ISSN: 1573-5141
DOI: 10.1007/bf00045956

No coin nor oath required. For personal study only.

✦ Synopsis

Development of techniques to maintain viable rotifer clones in a frozen state would preserve the genotype and reduce routine maintenance for those clones not being actively studied . To this end we have frozen Brachionus plicatilis in dimethyl sulfoxide at concentrations ranging from 6% to 18% . Survival rates decreased as the endpoint temperature was reduced from -20 °C to -45 °C, but did not decrease when the temperature was further reduced to -196 ° C (liquid nitrogen) . Only 2% of the individuals survived freezing in liquid nitrogen .

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