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Cryopreservation of Immature Bovine Oocytes to Reconstruct Artificial Gametes by Germinal Vesicle Transplantation

✍ Scribed by AM Luciano; F Franciosi; V Lodde; F Perazzoli; M Slezáková; S Modina


Book ID
108922129
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
259 KB
Volume
44
Category
Article
ISSN
0936-6768

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The effects of osmotic stress on germinal vesicle (GV) and metaphase II (MII) stage bovine cumulus oocyte complexes (COCs) were evaluated by first exposing them to various anisotonic NaCl solutions (75, 150, 600, 1200, 2400, and 4800 Ϯ 5 mOsm/kg) for 10 min and then returning them to isotonic TL-Hep