Cryopreservation of apices ofin vitroplantlets of sugarcane (Saccharumsp. hybrids) using encapsulation/dehydration

✍ Scribed by Florence Paulet; Florent Engelmann; Jean-Christophe Glaszmann

• Book ID: 104676430
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 585 KB
• Volume: 12
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00236101

✦ Synopsis

A cryopreservation process using encapsulation/dehydration was set up for apices sampled on in vitro plantlets of sugarcane. After dissection, apices were cultured for one day on standard medium and then encapsulated in medium with 3% alginate. Optimal conditions comprised preculture for 2 days in liquid medium with 250 g.l(-1) sucrose, desiccation for 6 hours under the laminar flow or for 10-11 hours with silicagel followed by rapid freezing and slow thawing. Survival after freezing in liquid nitrogen ranged between 38 and 91% for the 5 varieties experimented. Cryopreservation did not modify the electrophoretic profiles for aminoleucine peptidases and amylases with plants of the variety Co 6415.