Stable transformation of alfalfa (Medicago sativa L.) was obtained by particle bombardment of calli (derived from petiole and stem sections cultured for three weeks on SH medium), followed by delayed selection with 50 mg/l kanamycin on BOi2Y medium. Selection at a lower level of kanamycin (25 mg/l)
Cryopreservation of alfalfa (Medicago sativaL.)cells by encapsulation-dehydration
โ Scribed by R. Shibli; D. Haagenson; S. Cunningham; W. Berg; J. Volenec
- Publisher
- Springer
- Year
- 2001
- Tongue
- English
- Weight
- 45 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Diploid alfalfa (HG2), capable of plant regeneration from tissue culture, was used to select variant cell lines resistant to growth inhibition due to ethionine (an analog of methionine). Approximately 10(7) suspension-cultured cells were mutagenized with methane sulfonic acid ethylester and then pla
Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are sel