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Cryokinetic studies of the intermediates in the mechanism of carboxypeptidase A

โœ Scribed by Galdes, Alphonse; Auld, David S.; Vallee, Bert L.


Book ID
126951663
Publisher
American Chemical Society
Year
1983
Tongue
English
Weight
759 KB
Volume
22
Category
Article
ISSN
0006-2960

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Carboxypeptidase A (CPA), and other zinc-dependent proteases, facilitate an alpha deprotonation of judiciously designed ketones and amides. This adventitious reaction has been used in the development of effective mechanism-based inactivators for this family of enzymes. N-Acryloyl-L-phenylalanine, an