Cryo-electron Tomography of Whole Cells : Three-dimensional Mapping of Intact Cellular Architecture

✍ Scribed by Marek Cyrklaff; Petr Chlanda; Anna Sartori; Simone Lepper

• Book ID: 102866602
• Publisher: Wiley (John Wiley & Sons)
• Year: 2007
• Weight: 990 KB
• Volume: 9
• Category: Article
• ISSN: 1439-4243
• DOI: 10.1002/imic.200790182

✦ Synopsis

Three-dimensional Mapping of Intact Cellular Architecture

Cryo-electron tomography was used for studying the molecular organization of the cytoplasm in intact cells. Flat areas of cells grown on EM grids and preserved by rapid freezing within milliseconds were imaged in 3D. In the resulting maps the selected morphological and structural features were analyzed at unprecedented detail, and frequently correlated to light-microscopy images. We portray here host cell-virus interactions, the morphology of neurons and malaria parasites.