Crustacean hyperglycemic hormone in the lobster nervous system: Localization and release from cells in the subesophageal ganglion and thoracic second roots
✍ Scribed by Chang, Ernest S.; Chang, Sharon A.; Beltz, Barbara S.; Kravitz, Edward A.
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 446 KB
- Volume
- 414
- Category
- Article
- ISSN
- 0021-9967
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✦ Synopsis
Crustacean hyperglycemic hormones (CHHs) are neuropeptides involved in the regulation of hemolymph glucose. The primary source of CHHs has been identified as the neurosecretory neurons of the eyestalk X-organ and its associated neurohemal organ, the sinus gland. We have identified another source of CHH-like peptides in the nervous system. With the use of immunocytochemistry, cells in the second roots of the thoracic ganglia have been observed to stain positively for CHH-reactive material. We also identified a pair of cells in the subesophageal ganglion that contain large amounts of CHH-reactive material. Depolarization of these cells with elevated potassium mediates a calcium-dependent release of CHH-like material from the ganglion as quantified with an enzyme-linked immunosorbent assay (ELISA).