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Crucial Role of Conserved Lysine 277 in the Fidelity of tRNA Aminoacylation by Escherichia coli Valyl-tRNA Synthetase †

✍ Scribed by Hountondji, Codjo; Lazennec, Christine; Beauvallet, Christian; Dessen, Philippe; Pernollet, Jean-Claude; Plateau, Pierre; Blanquet, Sylvain


Book ID
125952732
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
233 KB
Volume
41
Category
Article
ISSN
0006-2960

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Spontaneous revertants of a temperature-sensitive Escherichia coli strain harboring a thermolabile leucyl-tRNA synthetase and seryl-tRNA synthetase were selected for growth at 40 degrees C. Among these, strains were found with increased levels of both thermolabile synthetases. Two distinct genetic l